Naslov

Hyaluronic acid enhances cell migration, viability and mineralized tissue specific genes in cementoblasts

Objective

Methods

Cementoblasts (OCCM-30) were treated with various dilutions ( from 1:2 to 1:128) of HA and examined for cell viability, migration, mineralization, and gene expressions. The mRNA expressions of osteocalcin(OCN), runt-related transcription factor 2(Runx2), bone sialoprotein(BSP), collagen type I(COL-I), alkaline phosphatase(ALP), cementum protein-1(CEMP-1), cementum attachment protein(CAP) and small mothers against decapentaplegic(Smad) -1,2,3,6,7, β-catenin(Ctnnb1) were performed with RT-PCR.

Results

HA enhanced cell viability and migration of the cells to the wound healing area. Increased mineralized nodules were determined at all HA dilutions. mRNA expressions of COL-I, BSP, RunX2, ALP, OCN, CEMP-1 and CAP significantly up-regulated by HA treatments compared with control group on day 3 and day 8. Smad 2, Smad 3, Smad 7 and β-catenin mRNAs were increased, while Smad1 and Smad 6 were not affected by HA administration.

Conclusions

Positive effects of HA on the cementoblast functions demonstrated that HA application may play a key role in cementum regeneration.

Funding source(s)

Selcuk University Research Coordination Office

Sažetak

Naslov

Complex treatment of gummy smile(GS) by periodontal subtractive surgery and esthetic zirconia crowns.

Objective

Methods

A young female patient is diagnosed with GS. The etiology of GS is related to altered passive/active eruption resulting in short tooth syndrome(STS). After facial, lip, and tooth analyses a digital wax-up and an indirect mock-up is tried on. Surgical crown lengthening is performed by apically positioned flap, osteoplasty, and otectomy using a surgical guide. The level of the osteotomy respects the distance of at least 2 mm from the apical ridge of the surgical guide on the maxilla. Three weeks later early preparation of teeth is performed by using a supragingival knife edge technique and healed soft tissue as a margin. Temporary crowns are applied. Six months later final zirconia crowns are cemented.

Results

Correction of GS is achieved and healthy periodontal tissues blend nicely with zirconia crowns

Conclusions

Analysis to determine the etiology of the GS must be done. The final position of restorations must lead the periodontist while performing the apically positioned flap along with osteoplasty and ostectomy(restorative approach) in order to establish healthy supracrestal tissues, even gingival margins, and proportioned teeth.

Funding source(s)

n/a

Sažetak

Naslov

Evaluation of the Efficacy of Topically Applied Melatonin Gel as Adjunctive Therapy in Chronic Periodontitis

Objective

Methods

40 pt selected and divided into 2 groups. First 1.treated by SRP combined by intrapocket melatonin administration weekly for 1 month. Second 2gp.SRP combined with placebo injection intrapocket and followed up weekly till 1 month

Results

Noticeable significant reduction in all clinical parameter and in amount of GCF RANKL at different intervals baseline, 1 week, 2 week, 1 month, 3 months

Conclusions

The result proved the benefits of melatonin As a promoting adjunctive therapy in improving periodontal parameters

Funding source(s)

Nil

Sažetak

Naslov

Vitamin C mesotherapy versus topical application for gingival hyperpigmentation: a clinical and histopathological study

Objective

Methods

Twenty ASA (American Society of Anesthesiology) type 1 individuals of both genders with age range 18-40 years old with increased pigmentation in gingivae were included randomly allocated into two groups. *Group 1 [Vitamin C intra-mucosal injection (G1.Mesotherapy)]: intra-mucosal field injections of 1 ml Cevarol were done then, repeated 3 times with 1 week interval. *Group 2: Vitamin C topical gel (G2. Gel): Ascorbic acid 2-glucoside gel was applied once daily for 3 months. Clinical assessment was done at baseline, after 3 months and 6 months by including the following parameters: *Dummett-Gupta Oral Pigmentation Index (DOPI) 0, pink tissue; 1 = mild light brown tissue; 2= medium brown or 3= deep brown. *Satisfaction questionnaire *Melanin area fraction (MAF) detecting melanocytes

Results

1-DOPI Group 1 showed significant change in DOPI by time. 2- Patient satisfaction score: Group 1 showed higher cosmetic satisfaction. 3-MAF: The difference in MAF was insignificant between the two groups by the end of treatment period.

Conclusions

The topical vitamin C is a promising alternative technique to surgical melanin depigmentation techniques.

Funding source(s)

Personal fund by all authors

Sažetak

Naslov

Digitally Assisted Esthetic Crown Lengthening In The Management of Gummy Smile: A Randomized Controlled Trial.

Objective

Methods

Sixteen patients, who were diagnosed with altered passive eruption type 1B, participated in this randomized clinical trial. The subjects were divided into two groups. In the control group (n=8) the surgical procedure was planned by manual measurements and in the study group (n=8) the planning was digitally assisted and the procedure was carried out using a dual guide. The clinical parameters were assessed at 1 week, 2 weeks, 3 months and 6 months postoperatively.

Results

There was no statistically significant difference in terms of wound healing, pain scores and gingival margin stability between both groups at different time intervals (P=1), however there was a statistical difference between both groups in terms of operating time with the study group being significantly lower (P<0.001).

Conclusions

Digitally assisted ECL helps shorten the operating time and reduces the possibility of errors during measurements taking. This will be useful in helping practitioners achieve better results.

Funding source(s)

Self funded.

Sažetak

Naslov

Oral and Gut Microbial Profiling in Periodontitis and Parkinson’s Disease

Objective

Methods

Patients with Stage III, Grade B periodontitis with (PA+P) and without PA (P); and systemically and periodontally healthy individuals (HC) were enrolled. Clinical and periodontal parameters were recorded. Severity of motor functions of PA patients was scored. Unstimulated saliva samples and stool samples were collected. Next generation sequencing of 16S ribosomal RNA (V1-V3 regions) was performed.

Results

PA patients had mild to moderate motor dysfunction which indicated that oral hygiene was not inadequate in PA+P group. In saliva, there were statistically significant differences between HC and PA+P (p=0.001), HC and P (p=0.012), P and PA+P (p=0.013) in beta diversity. The correlation between HC and PA+PD (p=0.001) and, P and PA+P (p=0.002) was also detected. The microbial profiles of saliva and fecal samples were distinct. In stool, Aitchison distance metric analysis showed statistically significant differences in PA+P and P groups when compared to HC. However, there were no significant differences of differential abundance in the gut microbiome profiles of the study groups.

Conclusions

These data suggested that Parkinson’s disease modifies the oral microbiome in periodontitis independent of the gut microbiome.

Funding source(s)

This research was supported by a grant from the NIH/NIA (R01AG062496 to A. Kantarci).

Sažetak

Naslov

Parkinson's Disease is Positively Associated with Periodontal Inflammation

Objective

Methods

Patients with Stage III, Grade B periodontitis with (PA+P) and without PA (P); and systemically and periodontally healthy individuals (C) were enrolled. Clinical and periodontal parameters were recorded. GCF samples from deep and shallow sites were collected to measure the inflammatory and neurodegenerative targets (YKL-40, fractalkine, S100B, alpha-synuclein, tau, VCAM-1, BDNF, NfL).

Results

Periodontal parameters and GCF volume were significantly higher in the P and P+PA groups than in the control group. PA was associated with significantly increased BOP compared to P-alone (p<0.05), while other clinical parameters were similar between P and P+PA groups. In GCF, YKL-40 levels from deep sites were significantly higher in the P+PA (p = 0.0105) and P (p = 0.0011) than in the C group. Alpha-synuclein levels were also higher in the P+PA group compared to the C (p = 0.0052) and P (p = 0.0012). S100B was higher in the P+PA than in the C group (p = 0.0194).

Conclusions

The data suggested that PA is highly associated with increased periodontal inflammatory burden -bleeding upon probing and inflammatory markers- in parallel with PA-related neuroinflammation.

Funding source(s)

This research was supported by a grant from the NIH/NIA (R01AG062496 to A. Kantarci).

Sažetak

Naslov

The adjunctive effect of probiotics to nonsurgical treatment of chronic periodontitis: RCT

Objective

Methods

Microbiological and clinical evaluation were recorded in 20 sites of chronic periodontitis in 12 patients and followed up at 3 and 6 months using clinical attachment level, probing pocket depth, plaque index (PI), bleeding on probing, and microbiologically for Porphyromonas gingivalis (P. gingivalis) load. Patients meeting the inclusion criteria were scheduled within 1 week for two sessions of SRP. After SRP, oral hygiene measures were reassured, and sites were divided randomly into two groups 10 sites each. Group I received SRP only, Group II received SRP and subgingival delivery of 1 ml of L. reuteri suspension at baseline and 1, 2, and 4 weeks using blunt syringe. Periodontal pack was applied after the placement of the drug.

Results

We found noticeable variation between the two groups in all evaluation aspects at 3 and 6-month follow-up except PI at 6 months, there was no significant difference.

Conclusions

Results proved the antimicrobial benefit of L. reuteri in improving periodontal parameters. However, further long-term studies with large sample size are needed to evaluate this added value.

Funding source(s)

self-funding except PCR kit supplied by our institution

Sažetak

Naslov

Evaluation of the Efficacy of Topically Applied Melatonin Gel as Adjunctive Therapy in Chronic Periodontitis

Objective

Methods

Forty chronic periodontitis selected patients ,divided into two groups. Group I: included 20 chronic periodontitis patients, treated by conventional periodontal therapy SRP combined with intra- pocket application of Melatonin gel once weekly for 1 month begin application at the second week after initial therapy. Group II: included 20 chronic periodontitis patients, treated by SRP combined with the injection of placebo, weekly for one month. The periodontal parameters were recorded at baseline, 1week, 1and 3 months.Gingival crevicular fluid (GCF) samples were collected and a quantitative measurement of receptor activator nuclear K ligand (RANKL) was carried out by using Enzyme-Linked Immunosorbent Assay (ELISA) at baseline, 1week, 1 and 3 months.

Results

We found noticeable significant reduction in all clinical parameter and in the amount of GCF RANKL in group I in all evaluation period at 1 ,3-month over group II.

Conclusions

The results proved the benefit of Melatonin as a promising adjunctive therapy in improving periodontal parameters. However, further long-term studies with large sample size are needed

Funding source(s)

nil

Sažetak

Naslov

DIFFERENCES IN BACTERIOME OF DENTAL PLAQUE, SALIVA, AND TISSUE BIOPSIES IN ORAL CANCER

Objective

Methods

Samples of tumour tissue, adjacent tissue, dental plaque and saliva from cancer patients were subjected to microbial identification using MALDI-ToF-MS. Conventional PCR was used to investigate prevalence of Porphyromonas gingivalis. Selected samples were subjected to bacterial 16S rRNA gene Next generation sequencing.

Results

Veillonella, Haemophilus, Actinomyces and Streptococcus species were found in abundance on identification using MALDI-ToF MS. Pasteurella, Eikenella and Selenomonas were prevalent in tumour and plaque samples, while being absent from adjacent tissue. Fusobacterium pseudoperiodonticum, Fusobacterium nucleatum, Fusobacterium canifelinum, and Fusobacterium hwasookii were found in high prevalence. Desulfotomaculum and Clostridium had a higher distribution in plaque. Tenericutes phylum was absent in plaque. Fusobacterium nucleatum was found in higher quantities as compared to P. gingivalis.

Conclusions

A significant microbiome-level overlap was noted between oral cavity and oral cancer lesions, thereby emphasizing the significant role of periodontal health and its maintenance.

Funding source(s)

The study has been supported by grant from Indian Council of Medical Research vide letter number NCD/Adhoc/87/2020-21 dated 17/3/2021(5/4/2021 Oral Health/ 2021- NCD-II)

Sažetak

Naslov

Resolvin E1 and Maresin 1 differentially regulate Porphyromonas gingivalis LPS-induced macrophage polarization

Objective

Methods

Murine macrophage cells were cultured and treated with P. gingivalis LPS with or without RvE1 or MaR1. M1 and M2 phenotypes were characterized by flow cytometry using CD80, CD86, F480, and CD206. TNF-α, TGF-β1, IL-6, IL-10, IL-1β, and NfκB expressions were measured by qPCR. Pro-inflammatory and anti-inflammatory cytokines were measured in cell culture supernatants.

Results

F4/80+ CD11b+ cells were significantly increased in response to P. gingivalis LPS; MaR1 significantly prevented this expression (p<0.05) while RvE1 did not have any significant impact. In parallel, RvE1 and MaR1 prevented the P. gingivalis LPS-induced increases in the expressions of TNF-α, IL-6, and IL-1β (p<0.05) while the SPMs did not significantly impact the TGF-β1 and IL-10 expressions. MaR1 and RvE1 significantly prevented the TNF-α production induced by P. gingivalis LPS.

Conclusions

SPMs differentially prevented the M1 polarization induced by P. gingivalis LPS while they did not impact the M2 phenotype, suggesting a reduced pro-inflammatory activation.

Funding source(s)

Hacettepe University, Ankara , Turkiye Forsyth Institute, Cambridge, MA, USA

Sažetak

Naslov

Gingival abscess as an early presentation sign for diagnosis of Mucormycosis

Objective

Methods

We are hereby presenting three Cases presenting with gingival abscess as an early presentation of mucormycosis. All the three cases presented with gingival abscess with no significant finding on routine radiological examination. Blood Investigations were normal except for two patients were diabetic. On first day of presentation, no other swelling or heaviness in maxillary area was seen. CBCT findings and bone biopsy were suggestive of mucormycosis.

Results

All the 3 patients were found to have osteolytic changes in the maxilla. The smear sent from the maxillary sinus after KOH examination was negative in these cases. Still, considering the suspicious nature of these lesions, intraoral biopsy was done. The biopsy result came positive in both KOH mount as well as histopathological examination. This is followed by the aggressive debridement of the lesion in all these patients.

Conclusions

Periodontist can play very important role in early detection and management of the mucormycosis for good prognosis.

Funding source(s)

None

Sažetak

Naslov

Effectiveness of titanium platelet rich fibrin in treatment of gingival recession with noncarious cervical lesion

Objective

Methods

14 subjects with Miller Class I gingival recessions in maxillary canine and premolar area associated with non-carious cervical lesions were selected. Cervical lesion were treated with resin modified glass ionomer restoration. Subjects were treated with Coronally advanced flap and Titanium Platelet Rich Fibrin. Probing depth (PD), relative gingival recession (RGR), Relative clinical attachment level (CAL), non-carious cervical lesion height (CLH), Keratinized tissue width (KTW) and keratinized tissue thickness (KTT) were measured at baseline and 6 months. Dentin Hypersenstivity,patient esthetics by visual analog scale were measured at baseline, 3, and 6 months postoperatively.

Results

Subjects showed statistically significant reduction in gingival recession. There was stastically significant gain in clinical attachment level and keratinised tissue thickness. There was significant difference in patient centered outcome (dentin hypersensitivity, patient esthetic score).

Conclusions

Coronally Advanced flap along with titanium platelet rich fibrin is effective treatment modality for treatment of Gingival recession associated with non-carious cervical lesion.

Funding source(s)

None

Sažetak

Naslov

Mean Platelet Volume, Triglyceride/High-Density Lipoprotein Cholesterol and Salivary Interleukin-1β as Determinants for Periodontitis Grading

Objective

Methods

Two-hundred and forty individuals were included to the study. Eight groups were constituted: gingival health and gingivitis (on intact and reduced periodontium) and periodontitis (stages 1-4). Clinical, sociodemographic and anthropometric parameters were recorded. Complete blood and lipid profile analysis were made in serum. Interleukin (IL)-1β and matrix metalloproteinase (MMP)-8 levels were analyzed in serum and salivary samples with Enzyme Linked Immunosorbent Assay (ELISA).

Results

According to the findings of our study, salivary and serum IL-1β and MMP-8 levels increased with disease severity, high density and very low density lipoprotein cholesterol levels showed significant differences among the groups (p<0.01). Mean platelet volume, salivary IL-1β and Triglyceride/High-Density Lipoprotein Cholesterol have shown high classification function coefficients in discriminating the grades of periodontitis.

Conclusions

Correlations between anthropometric, clinical, salivary and serum parameters and differences among groups could be taken into consideration, especially in grade determination. Considering the biomarkers, which are not reported yet as grade determinants in the current classification, the findings of our study are original and antecedent.

Funding source(s)

This study was supported by the Coordination Unit of Scientific Investigations, Süleyman Demirel University, Isparta, Turkey (project number: TDK-2022-8700).

Sažetak

Naslov

A Non-Surgical Evaluation of Enamel Matrix Derivative as an Adjunctive Treatment for Isolated Residual Pockets

Objective

Methods

In this split-mouth clinical trial (NCT05823389), initial non-surgical periodontal therapy was administered to 35 adults with stage III periodontitis. Following a six-week reinforcement of oral hygiene, laser therapy was utilized for re-instrumentation of deep, isolated pockets (5-8 mm) with bleeding on probing in a 3 month visit. The experimental group was administered EMD, whereas the control group was not. After EMD application, CAL, PD, BOP, GI were evaluated three months later.

Results

Compared to baseline, there was a significant decrease in periodontal pocket depth (PPD), clinical attachment level (CAL), and gingival index (GI) in the test group (1.721.13mm, 1.681.14mm, and 0.961.13mm, respectively; p 0.05). In contrast, PPD and CAL decreased significantly in the control group (0.40.76mm and 0.360.81mm, respectively) compared to baseline (p 0.05), whereas there was no significant difference in GI (0.240.65mm) (p > 0.05).

Conclusions

As an adjunct to non-surgical periodontal therapy, EMD in combination with laser therapy substantially improved periodontal health by decreasing CAL and PPD. EMD demonstrates a viable treatment option, minimizing the need for surgery in some cases.

Funding source(s)

none

Sažetak

Naslov

Cytokine Gene Polymorphysims and Socio-Demographic Parameters in Patients with Periodontal Disease

Objective

Methods

One hundred and fourty four individuals with systemically healthy and periodontal disease were included in this cross-sectional study. Socio-demographic characteristics [education level, economic status, tooth brushing and interdental cleaning habits and body mass index (BMI) etc.] and clinical periodontal parameters were recorded. All of the individuals were categorized as periodontal status (gingivitis, periodontitis and periodontally healthy). The genotypes and allele frequencies of TNF-α-308, -238, IL1β-511, +3953, IL-6-174. -572 polymorphisms were performed PCR-RFLP technique.

Results

IL-6-174 [the risk allele 2 (G/T allele)-(CG/CT genotypes)] was associated with gingivitis. There were significantly positive correlations between PcGPs and clinical periodontal parameters (p<0.05). As the severity of periodontal disease, individuals with periodontitis had worse socio-demographic and clinical periodontal parameters. Furthermore, BMI was significantly correlated with poor oral hygiene, clinical periodontal parameters and PcGPs.

Conclusions

Further longitudinal studies with larger populations are needed to explain the association among PcGPs, socio-demographic and clinical parameters of periodontal disease.

Funding source(s)

TÜBİTAK (project number, number: 107S506) Ankara-TURKEY

Sažetak

Naslov

Vertical bone augmentation - Khoury technique with computer guided autogenous bone harvesting

Objective

Methods

Safecut method is the method that is utilizing digital technology for safely harvesting autologous bone block from the mandible The case is showing bone harvesting utilizing a guide in a scenario where nerve exposure was planned before due to lack of bone in the mandible. Utilizing Khoury technique and FGG the ridge was regenerated completely.

Results

8 mm of vertical bone augmentation was obtained. 3 implants were inserted into the vital bone. 4 year follow up is showing stable bone and no bone remodeling in the implant neck.

Conclusions

Vertical bone augmentation is a predictable method. Survival rate in augmented bone is similar to the native bone in loading conditions. Safecut technique can offer us a safe, predictable, planned and user friendly technique to harvest a bone block.

Funding source(s)

De Stavola et al. 2015, jomi De Stavola et al. 2017, PRD De Stavola and Tunckel, 2013, Jomi

Sažetak

Naslov

Electrolysed oxidising water as a novel mouthwash for viral transmission prevention, in vitro study

Objective

Methods

In order to evaluate the in-vitro viral infectivity potential, a cytopathic effect assay was conducted using a model system to examine both membrane-enveloped and non-enveloped viruses. Mouthwash active ingredients, including Chlorhexidine, Cetylpyridinium chloride, Ethanol (EtOH), Hydrogen peroxide, Sodium hypochlorite (NaOCl), and EOW were assessed. Additionally, cytotoxicity was evaluated on human oral keratinocytes.

Results

EOW outperformed other mouthwashes, by absolute prevention of infectiousness of both virus types within a clinically relevant 1-minute exposure time. Except for EtOH, all other mouthwashes effectively targeted enveloped viruses, whereas only chlorine-based mouthwashes (NaOCl and EOW) exhibited potency against more robust non-enveloped viruses. Notably, all mouthwashes except for EOW exhibited cell cytotoxicity.

Conclusions

EOW showed promising results for mitigating viral transmission in in-vitro conditions, while remaining safe to human cells. This novel rinse should be considered for clinical applications.

Funding source(s)

The work was supported by the Ministry of Higher Education, Science, and Technology, Republic of Slovenia, under grant number P3-0293.

Sažetak

Naslov

Association between stages of periodontal disease and different diagnostic biomarkers

Objective

Methods

Periodontal examination was performed at 245 patients. Serum, saliva and GCF samples were quantitatively analysed for inflammatory biomarkers (matrix metalloproteinase-8 (MMP-8), interleukin-8 (IL-8), prostaglandin E-2 (PGE2), surfactant protein D (SP-D)) prior to active periodontal treatment. Staging was performed according to the 2018 classification. Univariate statistical analysis of differences of the expression of biomarkers in blood, saliva and GCF at various stages of periodontitis have been done with Analysis-of-Variance or Kruskal-Wallis-Test (α=0.05).

Results

Patient presented with a mean number of 24 (±5) teeth. 4 patients were diagnosed with periodontitis stage II (1.6%), 138 with stage III (56.3%) and 103 with stage IV (42.0%). Levels of MMP-8 in serum and GCF, of PGE2 in GCF, and of IL-8 in saliva showed significant differences between periodontal stages (p<0.001). On the contrary serum levels of SP-D values were not differentially expressed among patients with different disease stages.

Conclusions

Levels of MMP-8 in serum and GCF, of PGE2 in GCF and of IL-8 in saliva showed significant association with the stage of periodontitis. Accordingly, these biomarkers reach relevance in the diagnosis of periodontitis.

Funding source(s)

The study was self-funded by the authors and their institution.

Sažetak

Naslov

One health concept: periodontal disease from an interspecies approach

Objective

Methods

A literature review regarding human periodontology versus small animal periodontology and facts about periodontal disease in wildlife is performed.

Results

Periodontal disease is found in small animals, dogs and cats, and also it was reported in wildlife. The incidence of periodontal disease is higher in dogs and cats than in humans. Diagnosis in all species follows the same principles. The therapeutic approach varies and is adapted to every specie.

Conclusions

Working together and exchanging ideas will enhance long-term results for both human and veterinary dentistry. Everything that was achieved in veterinary dentistry was achieved with the help of human dentists. Let us be united in one health concept for the benefit of our patients.

Funding source(s)

No funding source

Sažetak

Naslov

Ceramic Soft-Tissue Trimmer vs Scalpel Surgical Technique in Gingival Depigmentation: A Split-mouth Randomised Controlled Trial.

Objective

Methods

Eight patients who had GH in both arches were randomized into two groups. So that, each patient had GD by scalpel in one arch and Ceratip in the opposing. The patients were followed up for 3-months. The assessed parameters were pain levels, Treatment time, clinical outcomes, Patient satisfaction, and re-pigmentation rates.

Results

There was no statistically significant difference in all the measured parameters at different assessment sessions. However, the Scalpel had better initial clinical outcomes. while, Ceratip had less visible re-pigmentation, pain Scores, treatment time, along with more patient satisfaction.

Conclusions

Ceratip and scalpel had comparable results. Thus, Ceratip is an alternative technique for Gingival depigmentation owing to its simplicity, efficacy and its bloodless nature.

Funding source(s)

Self-funded

Sažetak

Naslov

Ceramic Soft-Tissue Trimmer vs Scalpel Surgical Technique in Gingival Depigmentation: A Split-mouth Randomised Controlled Trial.

Objective

Methods

Eight patients who had GH in both arches were randomized into two groups. So that, each patient had GD by scalpel in one arch and Ceratip in the opposing. The patients were followed up for 3-months. The assessed parameters were pain levels, Treatment time, clinical outcomes, Patient satisfaction, and re-pigmentation rates.

Results

There was no statistically significant difference in all the measured parameters at different assessment sessions. The Scalpel had better initial clinical outcomes, While Ceratip had less visible re-pigmentation, pain Scores, treatment time, along with more patient satisfaction.

Conclusions

Ceratip and scalpel had comparable results. Thus, Ceratip is a viable technique for gingival depigmentation due to its efficacy, simplicity, and bloodless nature.

Funding source(s)

Self-funded

Sažetak

Naslov

Impact of physical exercise on local inflammatory profile in humans and rats

Objective

Methods

Seventeen periodontitis patients (according to Classification) were randomly allocated to receive either subgingival instrumentation (SI) or PE (high-intensity interval training,7min/day,3x/week). Periodontal parameters and gingival crevicular samples were collected at the worst site (PPD>=4mm with attachment loss and bleeding on probing) at baseline and after 45 days. Also, 16 male Wistar rats received cotton ligatures around the lower first molars, which remained for 7 days. Then, either ligatures were removed for periodontal repair (n=8) or rats started PE (swimming 30min/day,5x/week;n=8) for 28 days, when gingival samples and mandibles were collected. For both studies, samples were evaluated using an inflammatory panel (Luminex).

Results

In humans, mean PPD reduction was 0.24+-0.2 for SI and 0.14+-0.2 for PE (p>0.05). In rats, bone loss was not significantly different between groups. In both studies, IL-1beta and VEGF were increased after PE (although only significant for rats). IL-4,-10,-1-ra,TNF and EGF were comparable.

Conclusions

In conclusion, VEGF seems to play a key role on the beneficial impact of PE on periodontitis.

Funding source(s)

Araucária Foundation, Curitiba, PR, Brazil and National Council for Scientific and Technological Development (CNPq), Brasília, DF, Brazil (PPSUS).

Sažetak

Naslov

Novel Approach of Interdental Guided Creeping Therapy (IGCT) for Augmentation of Interdental Papillae: Case Report

Objective

Methods

Papillary recession was treated using IGCT. Horizontal incision was performed beneath the apical end of interdental junctional epithelium followed by creating a horizontal blind tunnel at the level of palatal papilla. Then the tunnel was augmented to support the papillary level at its ultimate coronal reposition. Interdental papillary fill and visual analogue scale for pain and swelling were measured.

Results

Papillary fill was obtained with minimal post-operative pain.

Conclusions

Interdental Guided Creeping Therapy could be a promising novel minimally invasive technique for treating papillary recession with minimal trauma.

Funding source(s)

n/a

Sažetak

Naslov

Case report of periimplantitis treatment with the help of new techniques and materials

Objective

Methods

After full thickness flap elevation and granulation tissue removal, the implant surface was disinfected with Galvnosurge unit. The exposed implant surface was augmented with the GBR technique. A composite bone graft was used to graft the periimplant bone defects. The bone graft was covered with a resorbale form stable membrane, secured with resorbable pins. After two weeks of healing, a small flap dehiscence occurred which was left to heal by secondary intention. Two months before implant uncovering procedure, a free gingival graft was used to correct the scars resulting from the secondary healing, improve the soft tissue thickness and widen the keratinized periimplant mucosa. One year after the surgical treatment the case was finalized with a definitive screw retained crown.

Results

At six months recall appointment, improvements in the clinical and radiological periimplant parameters were evident.

Conclusions

The combination of implant surface disinfection performed with the Galvanosugre unit, GBR procedure soft tissue augmentation resulted in a successful outcome of the periimplantits treatment.

Funding source(s)

n/a

Sažetak

Naslov

LOCALIZED FIBROUS GINGIVAL HYPERPLASIA: A CASE REPORT

Objective

Methods

A 46-year-old male complained on aesthetics compromised by single mass in the upper left canine region that has been present for 7 years. There was no systemic disease or long-term medication use in the patient's medical history. He had a car accident and facial injury 15 years ago. Clinical examination showed a solitary fibrous pedunculated nodular growth (1.8cm × 1.5cm × 0,8cm) that was attached with the buccal gingiva, extending from distal of 23 to mesial of 25. On palpation, it was firm and insensitive but bleeding on probing was present. The growth was surgically removed and sent for histopathologic examination.

Results

Histological evaluation showed moderately cellular, collagenized fibrous connective tissue with chronic inflammatory infiltrate. Overlaying stratified squamous epithelium is partly keratinized, variable thicked, without cytological atypia and focally infiltrated with neutrophils and lymphocytes.

Conclusions

A normal anatomy of attached gingiva was restored in order to improve oral hygiene maintenance and aesthetics outcome in 1, 3, 6 months follow up.

Funding source(s)

n/a

Sažetak

Naslov

Specialized pro-resolving mediators reverse transcriptomic changes in oral squamous cell carcinoma cells mediated by periodontopathobionts

Objective

Methods

We used oral squamous cell carcinoma cells (SCC-25) and infected them with Porphyromonas gingivalis (P.g.). We tested resolvin E1 (RvE1), maresin 1 (MaR1), or their combination at 10nM and analyzed the full transcriptomic profile.

Results

There was an upregulation of 43 and a downregulation of 27 genes in response to MaR1, while RvE1 upregulated 25 and downregulated 15 genes compared to the control group. The combined use of the SPMs led to upregulating of 104 genes and downregulating of 37 genes. Among several pathways the SPMs impacted, the tumor necrosis factor receptor family was a major target.

Conclusions

These findings suggest that SPMs reverse some transcriptomic responses of OSCC following P.g.-infection.

Funding source(s)

n/a

Sažetak

Naslov

Titanium chip preparation for fluorescence in-situ hybridization (FISH) analysis of oral samples

Objective

Methods

68 T-formed titanium chips were inserted into untreated periodontal/peri-implant pockets of 10 patients with periodontitis. After different resting times (1, 2, 7, 14 days), chips were removed and embedded in Technovit (Kulzer). The thickness of the chips was measured by a laser triangulation displacement sensor (optoNCDT 1420, Micro Epsilon, Ortenburg, Germany) and the most prominent part was assigned the value zero for the subsequent measurements. Multiple measurements were performed until a residual thickness of 30 µm was reached.

Results

The residual thickness of the chips ranged between 25 and 35 µm. After further processing an oral biofilm was detectable on all titanium chips (n = 65) by microscopic analysis. 3 chips were lost because of crooked embedding.

Conclusions

A grinding and measurement technique could be established, that provides the possibility to reduce the thickness of titanium-chips to 30 µm.

Funding source(s)

This study was supported by BMBF “PROCEED”(ref. no. 13GW0414D)

Sažetak

Naslov

Salivary C-reactive protein following non-surgical periodontal therapy in patients with stage III and IV periodontitis

Objective

Methods

This study included 30 patients with generalized periodontitis stage III and IV with a minimum of 20 teeth. The study group (n=15) underwent supragingival and subgingival full-mouth therapy with oral hygiene instructions, while the control group (n=15) received only supragingival therapy with oral hygiene instructions. Salivary CRP was determined at the baseline and three months after the non-surgical periodontal therapy.

Results

Statistical analysis revealed a reduction in the values of salivary CRP in the test group after therapy with a statistically significant reduction (p<0,01), and in the control group (p<0,01) at 3 months compared to baseline. Salivary CRP was statistically significantly lower in the study group compared to the control group 3 months after therapy (p = 0.035).

Conclusions

The study revealed decreased values of salivary CRP following the non-surgical periodontal therapy. Additional studies are needed to establish the consistency of the values of salivary CRP and possible use as a biomarker for periodontitis.

Funding source(s)

n/a

Sažetak

Naslov

Protein binding specificity predicts initial oral bacteria attachment on the implant and tooth surface

Objective

Methods

Protein layer was formed (2h) using human saliva and the proteomic profile was evaluated by mass spectrometry. Bacterial adhesion (2h) and initial biofilm formation (24h) were evaluated using an in vitro polymicrobial model using human saliva as microbial inoculum and analyzed by 16s RNA sequencing.

Results

Among the 349 proteins adsorbed, some were unique to titanium (12), dentine (42), or enamel (3). Linear discriminant analysis (LDA) showed that specific proteins drive toward a unique profile for each substrate. Differences (p<0.05) in abundance levels of 28 bacterial species among the substrates at 2h were found. Higher alpha diversity was found for enamel and titanium at 24h, with increased levels of Fusobacterium, Prevotella, and Streptococcus species for all substrates. LDA analysis showed that enamel and dentine are more closely related in terms of the microbiome. Canonical correlation analysis showed that specific proteins are correlated to higher abundances of specific bacterial species, as prediction analysis.

Conclusions

Therefore, dental implants and tooth surfaces differ in terms of salivary protein adsorption which predicts higher accumulation of specific bacterial species.

Funding source(s)

n/a

Sažetak

Naslov

A novel pH-sensitive MAA-based film coating antimicrobial LbL system for enhancing soft tissue seal

Objective

Methods

Detailed multilayer coating characterization was performed by different approaches. Coating stability of MAA-based film was confirmed under neutral and acidic pH. Microbiology experiments was performed to uncover the broad-spectrum of drug against different bacteria. The effect of MAA-based film on cell behavior was evaluated by cells in monolayer and collagen matrix.

Results

MAA-based film instability in acidic environment over time was identified through changes in physical-chemical properties. The protective capacity of MAA-based film was also identified with a higher concentration of drug released at acidic pH up to 15 days. Quantitative and qualitative assessments demonstrated a strong antimicrobial efficacy of MAA-based film against polymicrobial biofilm. Similarly, the non-cytotoxic effect was confirmed over a sufficient period to measure the hazard potential.

Conclusions

MAA-based film is a dual-function transparent coating that promotes soft tissue seal and holds antibacterial activity. Conveniently, the film discloses a slight capacity of controlling the drug release under neutral condition.

Funding source(s)

The São Paulo Research Foundation - FAPESP, processes no.: 2021/12175-6; 2021/10762-1; 2021/09434-0; 2018/20719-3.

Sažetak

Naslov

Clinical evaluation of sodium hypochlorite/amino acids and cross-linked hyaluronic acid adjunctive to non-surgical periodontal treatment

Objective

Methods

Forty-eight patients diagnosed with stages II-III periodontitis were randomly treated with either SD (control) or SD plus adjunctive sodium hypochlorite/amino acid and xHyA gels (test). Primary outcome variable was probing depth (PD) reduction, clinical attachment level (CAL), bleeding on probing (BOP) and plaque index (PI) were secondary outcomes. The outcomes were assessed at baseline, at 3 and 6 months following therapy.

Results

At 6 months, the test group showed statistically significantly better results in terms of mean PD reduction (2.9 ± 0.4 vs 1.8 ± 0.6 mm, p<0.001). Mean CAL gain was statistically higher in the test compared to the control group (test: 2.6 ± 0.5 vs control: 1.6 ± 0.6 mm, p<0.001). Mean BOP and PI scores decreased statistically significant difference favouring the test group (p<0.001).

Conclusions

a) Both treatments resulted in statistically significant improvements in all evaluated clinical parameters b) The adjunctive subgingival application of sodium hypochlorite/amino acid and xHyA to SD yielded statistically significantly higher improvements compared to SD alone.

Funding source(s)

Lithuanian University of Health Sciences Regedent

Sažetak

Naslov

Hyaluronic acid impact on periodontal condition of second molars after third molars extraction: Retrospective study

Objective

Methods

Twenty-one patients diagnosed with stages II-III periodontitis were referred for 3rd molar extractions before periodontal treatment. Patients were randomly allocated into test (the post-extractional alveolus filled with xHyA) and control (after 3rd molar extraction a suture was affixed) groups. Clinical measurements: probing depth (PD), clinical attachment level (CAL), bleeding on probing (BoP) and plaque index (PI) were obtained before extraction, after 3 and 6 months.

Results

No statistically significant differences were observed between groups at baseline. After 3 and 6 months statistically significant differences were found in PD and CAL changes in both groups compared to baseline (p<0.001). When a comparison between test and control was made, statistically significant differences were detected in changes of PD and CAL at 6 months in favour for test group (p=0.012 and p=0.006, respectively). BOP and PI decreased significantly in both groups at both timepoints (p<0.05), however, no statistically significant difference was observed between the groups.

Conclusions

Adjunctive application of xHyA after 3rd molar extraction lead to significantly better clinical improvements at the distal aspect of 2nd molars.

Funding source(s)

n/a

Sažetak

Naslov

Guided oral biofilm recolonization: the spray cryotherapy approach.

Objective

Methods

Multispecies oral biofilms were grown on hydroxyapatite discs, then treated with CryoPen® X+ for 1, 2, or 3 freeze/thaw cycles. Biofilms without treatment served as control. Biofilms were split into two groups, one for collection and analysis directly after the cryotherapy, and the other for reincubation for 24hr to allow biofilm recovery. Biofilms then were analysed with v-qPCR, scanning electron microscopy (SEM) and a confocal laser microscopy (CLSM) to follow possible changes.

Results

Cryotreatment decreased the biofilm bacterial load with more treatment cycles. The v-qPCR results showed significantly better ecology of the recovered biofilms than the non-treated ones. Also, CLSM showed changes in the layers of the biofilms after applying the cryotherapy and SEM revealed dominance of the commensal species in the biofilms after recovery.

Conclusions

Cryotherapy represent a totally new approach in guiding oral biofilm recolonization after treatment, with selective eradication of pathobionts and maintaining oral commensals, without antimicrobial use.

Funding source(s)

This study was supported by grants from the Fund for Scientific Research, Belgium (FWO G0B2719N) and KU Leuven (C24/17/086). J. Ghesquière holds a PhD grant for Strategic Basic Research from the Research Foundation Flanders (FWO-1SA5719N).

Sažetak

Naslov

Kynurenine Pathway, Hypoxia and Oxidative Stress in Periodontitis

Objective

Methods

Saliva and serum samples were collected from 23 systemically healthy stage III periodontitis patients and 22 periodontally healthy control individuals. Salivary and serum TNF-α, HIF-1α, 8-OHdG levels were evaluated with ELISA, and salivary and serum KP metabolites were evaluated with Tandem Mass Spectrometry (LC-MS/MS) method.

Results

Salivary TNF-α, HIF-1α, 8-OHdG levels were significantly higher in periodontitis group (p<0.05); serum levels did not differ among groups. Serum and saliva TRP levels were significantly lower in the periodontitis group than in the control group (p<0.001). Salivary and serum KYN/TRP ratio were significantly higher in the periodontitis group than in the control group (p<0.012 and p<0.001; respectively). Salivary HIF-1α and 8-OHdG were significantly positively correlated with serum KYN/TRP ratio.

Conclusions

Our results support that periodontal inflammation might play a role in local and systemic tryptophan-kynurenine metabolism and local hypoxia and oxidative stress levels are related with the systemic kynurenine metabolism.

Funding source(s)

n/a

Sažetak

Naslov

Healing patterns in a smoker and a non-smoker patients undergoing CAF operations with CTG.

Objective

Methods

Two patients, sound with an insignificant medical history and a close age gap, have a gingival recession on their tooth number 23 (FDI). A coronally advanced flap using Zuchelli's technique with connective tissue graft was utilized to amend the recession areas. the healing patterns were clinically monitored and recorded.

Results

The Non-smoker patient experienced faster and proper healing. However, in the smoker patient, healing in the operation zone took longer. Additionally, tooth #24 (FDI) remained uncovered, and a line of fibrotic tissue formed distally, likely due to prolonged healing time and/or inflammatory response.

Conclusions

It is important to emphasize postoperative patient education, particularly focusing on the effects of smoking on healing tissues. In non-smoker patients, the healing procedure tends to be faster and more proper. However, in smoker patients, a longer healing time in the operation zone is observed due to the known factors associated with smoking. These factors can significantly hinder the healing process and increase the risk of complications.

Funding source(s)

n/a

Sažetak

Naslov

The Osteogenic Differentiation of Human Gingival Fibroblasts Affects Osteoclast Formation

Objective

Methods

GF(n=12) were cultured for one, two or three weeks with osteogenic medium. After 3 weeks peripheral blood mononuclear cells(PMBCs) were added to each condition and co-cultured for 3 more weeks. To assess the mineralization, Alizarin Red S staining, Alkaline phosphatase(ALP) activity, Calcium(Ca), and scanning electron microscopy(SEM) were performed(day 21), followed by the determination of the gene expression of TRAcP, IL-1β, TNF-α, RANKL, and OPG by quantitative PCR(day 21 and 35). Further, protein levels of TNF-α were measured using ELISA at days 28 and 35.

Results

ALP/DNA and Ca concentrations increased after 3 weeks of culturing GF in mineralization medium(p<0.05 and p<0.01, respectively). Mean area covered by mineral nodules was assessed by SEM and increased gradually per time duration(p<0.05) which was confirmed by Alizarin Red S staining. All investigated gene expression levels significantly increased over time except for OPG which decreased(p<0.001).

Conclusions

GF are able to differentiate into osteoblast-like cells and produce mineral nodules. Their degree of differentiation seems to reduce their osteoclast inducing capacity. This further indicates their potential to participate in the development, differentiation, remodeling, and repair of the periodontium.

Funding source(s)

n/a

Sažetak

Naslov

The influence of increased body mass on periodontitis: a preliminary, cross-sectional study

Objective

Methods

A detailed medical history was obtained from each subject (n = 40), including age, gender, smoking habits, oral hygiene. A detailed periodontal clinical examination and radiographic analysis was performed. The body mass was assesed using the BMI and WHR. The test group consisted of 20 subjects with a diagnosis of periodontitis, while the control group consisted of 20 periodontally-healthy subjects.

Results

This study showed that the large proportion of subjects with periodontitis had an increased BMI (80%) and 50% of subjects had an increased WHR. In the control group, only 25% of the subjects had an increased BMI and 20% of them had an increased WHR. The median BMI in the test group was higher by 3.75 and the WHR median was higher by 0.05, when compared to the control group. The quality of life regarding oral cavity health is higher in healthy individuals than in those with periodontitis.

Conclusions

BMI and WHR were increased in subjects with periodontitis compared with subjects in the control group.

Funding source(s)

n/a

Sažetak

Naslov

Treatment of a localized gingival recession with different consecutive mucogingival procedures: A case report

Objective

Methods

A 43-year-old systemically healthy male patient was referred to our clinic with the complaint of gingival recession. Clinical examination revealed a 7 mm Cairo RT-II type of recession in the vestibule of upper left canine with lack of keratinized tissue. Following non-surgical therapy, recession was treated with the Envelope technique using de-epithelialized GG. Patient brushed the operation area in the early healing period, resulting in 2 mm recession. 4 months later, a semilunar coronally advanced flap surgery was performed, but because of complications during the healing process, the recession increased to 3 mm. A third operation was performed 2 months later, using enamel matrix proteins in combination with the Envelope Technique using de-epithelialized GG again.

Results

Root coverage was achieved on this challenging case. One month after the last surgical procedure, the recession depth was 0.5 mm, and keratinized tissue width was 5 mm.

Conclusions

Among the different bilaminar techniques, the envelope technique appears to be safe and predictable method in treating localized gingival recessions.

Funding source(s)

n/a

Sažetak

Naslov

Oral Hypersensitivity Reactions: Clinical and Histopathological Manifestations

Objective

Methods

Six cases of oral hypersensitivity reactions with known contacts that exhibited discrete clinical and histopathologic patterns were identified.

Results

Major histocompatibility groups, differences in TCR and TLR organizations, and allergy tendencies are effective in the formation of the reactions. As histological general findings: edema in the epithelium, inflammatory cell exocytosis, interface inflammation, perivascular inflammation in the deep layers of the connective tissue, lymphoid follicle formations and sometimes granulomatous reaction can be found.

Conclusions

Clinical presentation of OHR is varied and often overlaps with other oral conditions, complicating their diagnosis and management. OHRs may affect the patient's quality of life and should be diagnosed and treated by clinicians.

Funding source(s)

n/a

Sažetak

Naslov

Matrix metalloproteinase-8 in the gingival tissue of patients with arrested periodontitis following implant placement.

Objective

Methods

GT at the implant site were harvested during the first (pre-operative) and second stage (post-operative) implant surgery along with clinical data and gingival crevicular fluid (GCF) of the adjacent tooth. GT were formalin-fixed and paraffin-embedded prior to the immunohistochemical analysis. GCF MMP-8 level were quantified using commercially available enzyme-linked immunosorbent assay kit.

Results

29 subjects were recruited (15 control, 14 test; mean age: 48.41; 12 female) but only 24 post-operative tissue were available for analysis. MMP-8 immunostaining was positive in all samples. No significant difference seen on the pre-operative and post-operative GCF MMP-8 level and the GT immunoreactive score (IRS) between groups. However, the IRS showed increment in both groups post-operatively. Intense staining of MMP-8 was also seen in submucosa region of 7 samples which were not detected pre-operatively. There was significant correlation between the IRS of gingival epithelium and lamina propria at both time-points.

Conclusions

Increased intensity of MMP-8 staining in gingival tissue following implant placement may have a role in determining the onset of peri-implantitis.

Funding source(s)

Malaysian Ministry of Higher Education’s Fundamental Research Grant Scheme (grant no. FP011-2019A).

Sažetak

Naslov

Assessment Methods Of Gingival Tissue Volume: A Systematic Review

Objective

Methods

Electronic evidence searches were conducted on CENTRAL, MEDLINE, EMBASE, Web of Science (Core Collection), ClinicalTrials.gov, and grey literature from 01 January 2012 until 25 May 2023 in clinical studies involving human adult participants. Hand-searching was also performed and all relevant data from eligible studies were extracted. Qualitative analysis was performed.

Results

Initial search resulted in 2041 studies after the removal of duplicates. Then, 30 studies were retrieved for full-text screening following titles and abstract screening. Data from the studies were collated into an evidence table and grouped according to the study design. This provided an overview of the different methods to assess gingival tissue volume. Most of the studies reported on the methods of assessing gingival tissue thickness such as visual assessment, probe transparency, transgingival probing, CBCT, and ultrasound. Different studies utilised different criteria to classify gingival tissue thickness.

Conclusions

This review identified a range of clinical approaches employed to assess VGT, with a lack of consistency that undermines the reliability of employing these methods in clinical settings.

Funding source(s)

None

Sažetak

Naslov

Treatment of gingival recessions by Modified Tunnel Technique with De-epithelialized gingival graft: A Case Report.

Objective

Methods

A 19-year-old systemically healthy female patient who referred to our clinic complaining of gingival recessions and dentin hypersensitivity revealed multiple gingival recessions in different areas of her mouth. Thin gingival biotype and incorrect/excessive brushing were considered as possible etiologic factors. Following correction of oral hygiene habits and non-surgical periodontal therapy, multiple mucogingival operations were planned. A total of 4 consecutive modified tunnel technique with de-epithelialized GG were performed and the patient was precisely followed during whole treatment process. No complications were encountered in post-operative controls.

Results

In total 10 gingival recessions in all quadrants were treated and completely covered. The mean recession depth was 1.6mm and keratinized tissue width was 1.4mm at baseline. The mean keratinized tissue width was 5.05 mm at 12 months.

Conclusions

De-epithelialized GG with MCAT technique is a predictable treatment method for covering multiple gingival recessions. De-epithelialized GG is a successful alternative in stabilizing quality and dimension of the tissue obtained in the recession area.

Funding source(s)

n/a

Sažetak

Naslov

Neopterin levels in the serum and saliva of patients with periodontitis

Objective

Methods

Saliva and serum samples were collected from 23 systemically healthy periodontitis patients and 23 periodontally healthy control individuals. Clinical periodontal parameters (plaque index (PI), probing pocket depth (PPD), gingival recession (GR), clinical attachment loss (CAL), and bleeding on probing (BOP)) and saliva flow rates were recorded. Salivary and serum neopterin and 7-dihydroneopterin (7NP) levels were evaluated with Tandem Mass Spectrometry (LC-MS/MS) method.

Results

The concentrations of free and total salivary neopterin were statistically significantly higher in the patients with periodontitis compared with control group (p<0.001). In periodontitis group 7NP saliva was also significantly higher than control (p<0.001). There was no significant difference in serum between periodontitis and control groups. 7NP levels in the saliva were significantly and positively correlated with all the clinical periodontal parameters (PI, PPD, BOP, and CAL; p<0.01)

Conclusions

Our results demonstrated that salivary concentrations of neopterin (free, total and 7NP) were associated with periodontitis. This study suggests that salivary neopterin concentrations may reflect local immune activation even in situations where no systemic activation can be detected.

Funding source(s)

n/a

Sažetak

Naslov

Treatment of a localized gingival recession with different consecutive mucogingival procedures: A case report

Objective

Methods

A 43-year-old systemically healthy male patient was referred to our clinic with the complaint of gingival recession. Clinical examination revealed a 7 mm Cairo RT-II type of recession in the vestibule of upper left canine with lack of keratinized tissue. Following non-surgical therapy, recession was treated with the Envelope technique using de-epithelialized GG. Patient brushed the operation area in the early healing period, resulting in 2 mm recession. Four months later, a semilunar coronally advanced flap surgery was performed, but because of complications during the healing process, the recession increased to 3 mm. A third operation was performed 2 months later, using enamel matrix proteins in combination with the Envelope Technique using de-epithelialized GG again.

Results

Root coverage was achieved on this challenging case. One month after the last surgical procedure, the recession depth was 0.5 mm and keratinized tissue width was 5 mm.

Conclusions

Among the different bilaminar techniques, the envelope technique appears to be safe and predictable method in treating localized gingival recessions.

Funding source(s)

n/a

Sažetak

Naslov

Continuous vs. intermittent maintenance - a 12-year clinical review.

Objective

Methods

The study examined 16 patients with adult periodontal disease who underwent a full-mouth radiographic examination before and 12 years after the initial examination. Tooth loss, probing pocket depth (PPD) change, and mean alveolar bone loss (MBL) over 12 years were evaluated. Based on the regularity of patients' visits in the phase of SPT, the patients were divided into two groups: continuous maintenance (n=8) and intermittent maintenance (n=8).

Results

In the group of continuous maintenance, the results show a 63,4% decrease in sites with PPD>46mm. The average PPD has decreased by 1,16mm, MBL regarding the initial exam was 1,23mm, and 2,1% of teeth were lost. In the group of intermittent maintenance, the results show 1.4x more sites with PPD>46mm. The average PPD has increased by 0,31mm, MBL regarding the initial exam was 2,41mm, and 11,8% of teeth were lost.

Conclusions

The results show a significant difference in disease progression between the two groups. The group of continuous maintenance has a significantly better outcome.

Funding source(s)

n/a

Sažetak

Naslov

Continuous vs. intermittent maintenance - a 12-year clinical review.

Objective

Methods

The study examined 16 patients with adult periodontitis who underwent a full-mouth radiographic examination before and 12 years after the initial examination. Tooth loss, probing pocket depth(PPD) change, and mean alveolar bone loss(MBL) over 12 years were evaluated. Based on the regularity of patients' visits in the phase of SPT, the patients were divided into two groups: continuous (n=8) and intermittent maintenance (n=8).

Results

In the group of continuous maintenance, the results show a 63,4% decrease in sites with PPD4-6mm and an 81,5% decrease in sites with PPD above 6mm. The average PPD has decreased by 1,16mm, MBL regarding the initial exam was 1,23mm, and 2,1% of teeth were lost. In the group of intermittent maintenance, the results show 1.4x more sites with PPD4-6mm and 4.7x more sites with PPD above 6mm. The average PPD has increased by 0,31mm, MBL regarding the initial exam was 2,41mm, and 11,8% of teeth were lost.

Conclusions

The results show a significant difference in disease progression between the two groups. The group of continuous maintenance has a significantly better outcome.

Funding source(s)

n/a

Sažetak

Naslov

Identification of Dental Implant Failure Risks, an overview of systematic reviews

Objective

Methods

We searched Epistemonikos, Cochrane Database of Systematic Reviews and PubMed. Authors (TPP, JV, NB, MM) independently screened search results using Covidence. Same authors will independently extract data from included SRs. Authors (TPP, JZ, NB, TG) working in pairs will independently evaluate the quality of SRs using AMSTAR 2. Size and direction of DIF risk for population, exposure, and outcome measures will be summarized.

Results

After deduplication, 299 records were screened, with 31 SRs preliminarily included. The following risk factors were assessed: loading protocol (9 SRs), HIV infection (1SR), implant surface (2 SR), presence of cantilevers (1SR), screw- or cement-retained prosthesis (1 SR), splinted vs non-splinted implant-supported restorations (1 SR), diabetes (3 SRs), use of bisphosphonates (3 SRs), history of periodontitis (3 SRs), implant length (1 SR), osteoporosis (1 SR), polymorphisms in IL-1 and TNF (1 SR), bone augmentation (1 SR), sex (1 SR), and bruxism (1 SR). One SR included smoking, radiotherapy, diabetes, and osteoporosis.

Conclusions

Understanding the likelihood of DIF regarding different risk factors and performing individual risk assessment before dental implant placement is crucial for the long-term success of implant rehabilitation.

Funding source(s)

No extramural funding.

Sažetak

Naslov

The effect of medical Manuka honey in the treatment of periodontitis- randomized clinical trial

Objective

Methods

In this randomized clinical trial, a total of 45 participants with stage III periodontitis underwent full-mouth non-surgical therapy. Manuka honey was applied after randomization to 23 paricipants, 22 participants recived placebo. The effectiveness of Manuka honey was investigated by measuring the clinical parameters of periodontal disease (bleeding on probing, probing depth, plaque index, recession) at two time-points, T0 and after 3 months. The microbiological analysis at 3 time-points, T0, after one week and after 3 months.

Results

Percentage of pocket closure: Manuka(M) 78.8% Placebo(P) 69.0% CAL gain(mm): M 0.87±1.47 P 0.67±1.35 PPD reduction(mm): M 1.07±1.4 P 0.87±1.28

Conclusions

The use of Manuka honey showed a promising potential for being used as an adjunctive therapy to NST. The improvements in outcomes were statistically significant for the participants treated with Manuka in terms of pocket closure, PPD reduction, CAL gain and reducation of bacteria associated with periodontitis after each of the follow-up time points.

Funding source(s)

n/a

Sažetak

Naslov

EPULIS: CLINICAL AND MORPHOLOGICAL FEATURES

Objective

Methods

Clinical examination and surgical removal of tumor-like gingival lesions was carried out in 17 patients aged 16 to 84 years. Histological study of all biopsy specimens was performed.

Results

Various forms of epulis were identified in 17 women, most of whom (70.6%) were young (up to 44 years). Fibrous epulis diagnosed in 9 cases (52.9%). In 6 women, of whom 4 were pregnant, vascular epulis, or pyogenic granuloma, was observed. Also, 2 women (61 and 84 years old) were with inflammatory fibrous hyperplasia (epulis fissuratum) that result from chronic trauma induced by a denture flange. Epulis is more common on the gingiva and alveolar mucosa of the maxilla (64.7%). Fibrous and vascular epulis are located near incisors and canines in 11 patients, premolars - in 2 women, molars also in 2 women.

Conclusions

To clarify the diagnosis of epulis, histological study of biopsy specimens is mandatory.

Funding source(s)

n/a

Sažetak

Naslov

Non-invasive periodontal examinations using ultrasonography and artificial intelligence automated processed images.

Objective

Methods

Examinations of pig mandibles, but also of periodontally healthy human patients or gingivitis and periodontitis patients having or not calculus deposits were performed using 20 – 40 MHz standard ultrasound machines having the transducer placed intraorally or extraoral. 3D ultrasound reconstruction of larger areas of the maxillary and mandibular alveolar process were performed using a 3D ultrasound scanner prototype for lowering operator dependence.

Results

3400 annotates periodontal ultrasound images were used for training artificial intelligence models for faster imaging diagnosis. The obtained IoU ranged between 40 and 80% corelated with the size of the masked tissue and the number of annotated images in which it appears.

Conclusions

3D Periodontal ultrasonography may help a less experienced operator to generate higher quality datasets in the future. To achieve a higher quality of the results for automatic segmentation, further studies are necessary using larger datasets for training AI models.

Funding source(s)

This paper was partially realized with the material, equipment, technology, and logistic support of Chifor Research SRL, through the project Periodontal ultrasonography in diagnosing and monitoring the periodontal disease (Chifor Research SRL, Operational Program Competitivity, Ministry of European Funds from Romania, P_38_930\12.10.2017, Project ID 113124.) This paper was partially funded by the European Social Fund, Human Capital Operational Program 2014–2020, project no. POCU/380/6/13/125171, EIT Health-RIS Innovation Program 2020, project ID 2020, RIS-1001-825

Sažetak

Naslov

Influence of Streptococcus salivarius on Aggregatibacter actinomycetemcomitans in Mixed Biofilm on dense polytetrafluoroethylene (d-PTFE) membranes

Objective

Methods

An early mixed streptococcal biofilm (Streptococcus mutans and Streptoccocus oralis) with and without Streptococcus salivarius K 12 was tested for its inclusion of Aggregatibacter actinomycetemcomitans in the mature mixed biofilm by determination of the number of bacteria on both tested membranes in salivarius and non-salivarius mixed biofilm. At the same time, the presence of biofilm matrix production in the mixed biofilm was monitored by using the fluorescent staining method.

Results

The total number of bacteria was greater in the salivarius mixed biofilm, with a significant difference on the Permamem® membrane, but the number and the proportion of Aggregatibacter actinomycetemcomitans in the mature 72-h biofilm was significantly lower in the salivarius mixed biofilm regardless of the membrane tested. The presence of Streptococcus salivarius increased the biofilm’s biomass due to the creation of an EPS-rich matrix and the development of a complex biofilm structure.

Conclusions

Streptococcus salivarius inhibited the growth of Aggregatibacter actinomycetemcomitans in the mixed mature biofilm and helped maintain the physiological balance in the oral biofilm.

Funding source(s)

n/a

Sažetak

Naslov

IMPACT OF HYALURONIC ACID ON FREE GINGIVAL GRAFT SHRINKAGE AND KERATINIZED TISSUE GAIN: PILOT STUDY

Objective

Methods

14 patients with keratinized tissue width less than 2 mm underwent the surgical procedure. The patiens were randomly, examiner blind divided in two groups: HA and control. Difference between the groups were analysed (ANOVA, post-hoc Bonfferoni, Mann-Whitney U test).

Results

The graft areas were measured in horizontal and vertical dimensions on baseline, 14 days, 1 month, 3 months and 6 months postoperatively. Shrinkage of graft was higher in control group during time (19.10±5.46, p<0.1). There was no statistically significant difference between groups at the same time point. Keratinized tissue gain was statistically significant between the groups 3 months postoperatively (p=0.01). In HA group there was significantly better result of keratinized tissue gain during the whole study period. Changes in gingival recession did not present statistically relevant results in this study.

Conclusions

Seems that HA might have positive effects on graft area dimensional changes and keratinized tissue gain pointing to the potential clinical relevance in keratinized tissue augmentation procedures.

Funding source(s)

n/a

Sažetak

Naslov

Lack of physical activity as a risk factor for severe periodontitis. A cross-sectional pilot study.

Objective

Methods

SP patients (stage III,IV) were considered as cases and mild periodontitis (MP) patients (stage I,II) were considered as controls. Along with anamnesis and periodontal examination, patients were given GPAQ questionnaire and underwent Six Minute Walk test (6MWT) for assessment of physical activity. The object of this test was to walk as far as possible for six minutes and total distance was recorded.

Results

Out of 50 patients, 38 were diagnosed as SP and 12 had MP. SP were less physically active according to GPAQ (58.3% vs. 72.7%) whereas they had similar scores of 6MWT; both results did not show statistically significant difference (P=0.613, P=0.674). Logistic regression analysis adjusted for age, gender, smoking and body mass index showed that LPA might be associated with increased risk for SP. This finding did not reach statistical significance based on this sample size.

Conclusions

In this pilot study, LPA was not statistically significant predictor of SP. These results should be confirmed on the larger sample size.

Funding source(s)

None.

Sažetak

Naslov

Papillon-Lefèvre Syndrome in an 8-Year-Old Patient: A Case Report

Objective

Methods

An 8-year-old patient with PLS was evaluated at a university clinic. The examination involved a detailed medical and dental history, intraoral and extraoral assessments, radiographic examination, and genetic testing. The patient showed extensive periodontal destruction, including deep pockets, bleeding, and significant loss of clinical attachment. Radiographs confirmed alveolar bone loss. Treatment included periodontal therapy, consisting of oral hygiene instruction, scaling and root planing for bacterial infection control, and systemic antibiotics for inflammation.

Results

After treatment, there was a reduction in inflammation, with improved gingival health and reduced bleeding. However, due to the advanced disease stage at diagnosis, the patient experienced premature loss of several primary teeth.

Conclusions

Early diagnosis and periodontal therapy are crucial for managing PLS in pediatric patients. The condition can lead to severe periodontal destruction and early tooth loss, necessitating prompt and tailored interventions. Collaboration between dentists and medical specialists is vital to provide comprehensive care and enhance long-term oral health outcomes for affected individuals.

Funding source(s)

n/a

Sažetak

Naslov

MIF promotes periodontal disease associated to pregnancy in a murine model

Objective

Methods

In this work we evaluated in a murine model of pregestational PD in BALB/c females WT and MIF-/- mice, histological changes and histometric analysis of the clinical attachment loss (CAL), relative expression of metalloproteinase (MMP)-2 and -13 by immunofluorescence and the relative expression of mif, tnf-α, ifn-γ and il-17 by qPCR.

Results

We found elevated levels of MMP-13, mif, tnf-α. Theses correlated with tissue destruction and increased depth of the CAL in PGPD WT mice compared with control. Importantly we found over expression of MMP-2 and -13 and il-17, abated levels of tnf-α, ifn-γ and a severe tissue remodeling and no CAL modification in PGPD Mif-/- mice.

Conclusions

These results suggest MIF plays an important role in exacerbating the inflammatory pathology of preexisting periodontitis. The absence of MIF down regulates inflammatory transcripts and decreases insertion loss in PD.

Funding source(s)

Consejo Mexiquense de Ciencia y Tecnología (COMECYT) FICDTEM-2021-072 and PAPIIT-UNAM IN-217021

Sažetak

Naslov

How does periodontal treatment affect quality of life; A case series

Objective

Methods

At the initial clinical and radiographic examinations, 4 patients who are diagnosed with Stage 3, Grade B/C periodontitis are selected. Patients were asked information about demographic, social-economic status, and oral hygiene practice. Clinical periodontal parameters were evaluated before and 4weeks after NSPT. OHIP-49 TR questionnaire filled by patients to assess their oral health quality of life before and 4weeks after NSPT.

Results

Clinical periodontal parameters and OHIP-49 TR scores have shown a significant change 4weeks after NSPT.

Conclusions

NSPT not only leads to a significant enhancement in both periodontal health but also the overall quality of life.

Funding source(s)

n/a

Sažetak

Naslov

Different porositiy sizes of titanium meshes influence bone neoformation in GBR: microtomographic and histomorphometric study

Objective

Methods

Five milimeters in diameter defects were created in calvaria of 36 rats, that were randomly divided into six groups (6 animals per group): NCOG (negative control), TEMG (Teflon membrane), SPTMG (Small pore titanium mesh), SPMMG (Small pore mesh +Teflon), LPTMG (Large pore titanium mesh), LPMMG (Large pore mesh +Teflon). After 60 days, the animals were sacrificed, and the bone tissue formed was evaluated. The data were compared by ANOVA followed by Tukey post-test (p≤0.05).

Results

Microtomographic results showed that the GMMP group presented the highest value for BV/TV (22.24±8.97), with statistically significant differences for all the other groups except GMMG. Considering the histomorphometric evaluation, groups with only porous titanium meshes showed higher values compared to the groups that used the Teflon membrane and the negative control. SPTMG presented higher values in the parameters of area (0.44 mm²±0.06), extension (1.19 mm²±0.12), and percentage (7.56%±1.45) of neoformed bone.

Conclusions

Titanium mesh with smaller pores showed better results, suggesting a correlation between mesh porosity and underlying bone repair.

Funding source(s)

n/a

Sažetak

Naslov

Interdisciplinary treatment of endo-perio lesions

Objective

Methods

Medical history of a 24-year-old male revealed a presence of a deep narrow pocket on the tooth 25, on which root canal treatment (RCT) was previously performed. The patient was referred to an endodontist for a second opinion due to suspected vertical fracture. Clinical and radiographic examination confirmed the diagnosis of endo-perio lesion. Comprehensive treatment included non-surgical RCT, followed by root scaling and planning of the pocket to eliminate infection and inflammation.

Results

After one week, the narrow deep pocket closed. The success of treatment was monitored clinically by the presence of the pocket and radiographically by the size and presence of a periapical lesion. A 6-month follow-up showed no signs of pathology.

Conclusions

Non-surgical periodontal therapy combined with RCT successfully resolved the endo-perio lesion of the tooth 25, avoiding extraction. This case emphasizes the significance of a multidisciplinary approach in managing such cases.

Funding source(s)

n/a

Sažetak

Naslov

Pink tooth - Cervical external root resorption

Objective

Methods

Medical history of a 20-year-old male undergoing orthodontic treatment revealed a pink coloration in the cervical region of the tooth 11. The patient was referred to endodontist. Clinical and radiographical examination confirmed CRR. Considering the tooth’s vitality we opted for minimal invasive treatment. Papilla-preserving flap was performed under local anesthesia, followed by removal of granulating tissue using diamond and carbon steel burs. The cavity was thoroughly disinfected, followed by adhesive bonded restoration with composite. After polishing of the restoration, the flap was secured with suturing.

Results

After one year, the patient remained asymptomatic, with a vital tooth showing no tenderness to percussion and no increased probing depth. Radiographically, no evidence of radiolucency was observed.

Conclusions

Regular monitoring of orthodontic patients is crucial for early detection of CRR. Despite its asymptomatic nature, early detection and treatment are essential to avoid endodontic treatment or more severe complications such as increased tooth mobility or tooth loss.

Funding source(s)

n/a

Sažetak

Naslov

EXPERIENCE IN THE USE OF HYALURONIC ACID IN THE TREATMENT OF PERIODONTAL POCKETS

Objective

Methods

22 patients with periodontitis II degree aged 34-45 participated in clinical studies. 12 patients made up the experimental group using hyaluronic acid "Hyadeny BG" (BioScience, Germany), 10 patients - the control group. In each group, conservative treatment was performed (scaling root planning). PD was re-measured after 3 weeks. In 12 patients of the experimental group, after SRP, 0.1 ml of "Hyadent BG" was injected into PD of 5-7 mm. In the comparison group, only repeated SRP was performed.

Results

The pocket depth in patients of the experimental group before treatment was 5.82 ± 0.97 mm, in the control group - 5.78 ± 0.86 mm. 3 months after treatment, the PC depth in the experimental group was 5.02 ± 1.09 mm. PD after treatment in the control group was 5.47 ± 0.94 mm.

Conclusions

The use of the drug Hyadent BG is promising in the treatment of periodontal pockets in patients with generalized periodontitis, which is due to the pronounced regenerative properties of hyaluronic acid.

Funding source(s)

n/a

Sažetak

Naslov

Effectiveness of minimally invasive non-surgical approach in the treatment of severe periodontitis: A prospective study

Objective

Methods

Thirteen patients with 31 defects were included in the study. The teeth were treated by sub-papillary access to the defect, with the aid of magnification loupes. Thorough debridement of the root surfaces is conducted with Gracey minicurets under local anesthetic. Following debridement, a stable clot was stimulated by natural filling of the defect with blood. Chlorhexidine digluconate was used twice a day for 2 weeks, and regular supportive periodontal therapy was performed monthly.

Results

At 6 months posttreatment PPD reductions was 4.37 ± 0.76 mm, with all the patients achieving a PPD of ≤ 5 mm in the treated site. CAL gains was was 3.34 ± 2.39 mm. A statistically significant bone gain of 2.91  ± 2.31 mm was reported.

Conclusions

Treatment of periodontally compromised teeth with MINST with subsequent application of a meticulous maintenance protocol can be a viable alternative for invasive procedures. Further studies are needed to evaluate the long-term outcomes achievable with MINST.

Funding source(s)

None

Sažetak

Naslov

Development and in vitro evaluation of doxycycline and atorvastatin loaded alginate nanoparticles for periodontal pockets

Objective

Methods

To develop this dual drug delivery system, ionic gelation method was used to synthesized AS and DS loaded alginate nanoparticles (AS/DS-ALG) at various drug weights, such as DS:AS ratio percent w/w, 100/0, 0/100, and 50/50. FTIR spectroscopy and scanning electron microscopy (SEM) were used to determine the chemical structure and surface morphology of the nanoparticles, respectively. The amount of AS and DS from in vitro release studies were determined by HPLC method in artificial saliva medium at 37 °C. In vitro antimicrobial studies performed against Porhyromonas gingivalis, Eschericha coli, and Staphylococcus aureus. antimicrobial studies performed against Porhyromonas gingivalis, Eschericha coli and Staphylococcus aureus.

Results

The chemical structure of AS/DS-ALG was confirmed by FTIR. The distribution of particle size was narrow and uniform, which was measured by SEM. Inhibition zones for AS/DS nanoparticles were measured as 11±1, 15±2 and 37±4 mm for E. coli, S. aureus and P. gingivalis, respectively.

Conclusions

The AS- and DS-loaded alginate drug delivery system has been developed as a promising adjunctive therapeutic candidate for periodontal pockets, exhibiting noteworthy in vitro antimicrobial efficacy.

Funding source(s)

n/a

Sažetak

Naslov

Smoking Thirties: How Tobacco & BMI Shape the Subgingival Microbiome

Objective

Methods

183 subjects were recruited following informed consent and divided into groups based on body mass index and smoking status. Bacterial 16S rDNA genes were sequenced from subgingival plaque samples. Sequence analyses were conducted using QIIME and PhyloToAST. Metabolomic analysis was performed on saliva samples using NMR spectroscopy. Levels of selected cytokines and adipokines in gingival crevicular fluid were determined by multiplexed bead-based assay.

Results

Linear Discriminant Analysis revealed significant group separation between obese smokers and normalweight nonsmokers (p =0.001). Obese smokers were enriched for putative pathogens belonging to the genera Atopobium, Bacteroidaceae, Bifidobacterium, Dialister, Lactobacillus, Mycoplasma, Prevotella, Treponema and Veillonellaceae. Salivary metabolic profiles also differed significantly between obese smokers and normalweight nonsmokers. Obese smokers presented with higher levels of lactate acid and lower levels of methanol compared to normalweight nonsmokers. Significant correlations were observed between these metabolites and bacterial community networks.

Conclusions

obesity and smoking impact the subgingival microbiome in significant and distinct ways, leading to enrichment of specific bacterial species, as well as the metabolites produced by them.

Funding source(s)

n/a

Sažetak

Naslov

Predicting Periodontitis Severity and Complexity from periapical x-rays using deep learning networks

Objective

Methods

Standardized Periapical radiographs were taken for periodontitis patients at Jordan University Hospital. -Total of 2643 radiographs were collected from 143 patients, excluding poor quality radiographs, resulting in 2432 radiographs. -Radiographs were categorized into; training set, validation set, and testing. -Annotation for the training set was done by labeling bone, root, and crown for the segmentation model. -Image analysis model was used to calculate RBL% and determine type of BL. -For FI, object detection models were used. 124389 radiographs were collected, and radiographs with no FI were excluded resulting in 1500 radiographs.

Results

-Accuracy for segmentation model was 92% -Accuracy for detection of FI was 90% (mandibular) and 60% (maxillary). -Accuracy of bone loss differentiation was 88% -Model can accurately determine the stage of periodontitis according to the amount of RBL.

Conclusions

-The deep learning model can accurately predict the severity (RBL%) and complexity (FI and type of bony defect) using periapical x-rays for periodontitis patients.

Funding source(s)

University of Jordan research fund

Sažetak

Naslov

NON-SURGICAL PERIODONTAL THERAPY IN A PATIENT WITH “BURNOUT SYNDROME”

Objective

Methods

A 49-year-old systemically healthy female, smoker (15 cigarettes/day), was referred for teeth mobility and significant gingival hyperplasia. The patient reported subjective depression and burnout syndrome-related symptoms, which were not professionally managed or treated. The patient was diagnosed with generalized periodontitis, stage IV, grade C. Treatment followed the evidence-based guidelines. After steps I and II, the patient was prescribed systemic antibiotics. She was also further referred to a restorative dentistry specialist.

Results

A significant improvement in clinical presentation and all periodontal indices was observed at reevaluation. The patient’s oral hygiene habits significantly improved and reported reduced work-related stress. She received further localized corrective treatment (step III) and was enrolled in supportive therapy (step IV).

Conclusions

A successful long-term outcome relies on cause-related treatment and systemic risk factor control, including stress. The complex interactions of stress and depression in inflammatory periodontal diseases remain to be further studied and defined.

Funding source(s)

n/a

Sažetak

Naslov

THREE FREE GINGIVAL GRAFT TECNIQUES FOR LOWER ANTERIOR INCISOR RECESSIONS

Objective

Methods

Patients were systemically healthy non-smokers. Three surgical techniques are 1-stage conventional FGG, 2-stage FGG + later CAF, and a novel, modified FGG. After the respective recipient site preparation, a graft was harvested from the palate region, averaging 1.5 mm in thickness and sutured at the recipient site with 5-0 and 6-0 sutures. In the case of the 2-stage approach, the coronal advancement was performed 3 months following the first surgery.

Results

Sutures were removed 2 weeks post-op. The healing was appropriate in all patients at 2 weeks and at 3 months follow-up. An increase in KT was observed with all 3 techniques, however, root coverage varied. All sites presented with chromatic and tissue texture changes.

Conclusions

FGG remains one of the surgical treatment options, particularly in the lower anterior regions. Modified FGG may improve root coverage and, thus, decrease the need for second-stage surgery.

Funding source(s)

n/a

Sažetak

Naslov

Short implants as an alternative treatment for areas of implant explantation.

Objective

Methods

Three patients complaining of implant related problems referred to Ege University, Faculty of Dentistry, department of periodontology were clinically and radiologically examined(All male were in their 50's, systemically healthy with no allergies or other medical conditions).The implants were in function for more than 10 years and patients didn't comply to recalls. Three implants placed in the patient had bone loss; two implants because of periimplantitis and one implant because of an implant breakage. All the implants were explanted and after a healing period of 6 week one short implant for each explanted implant were placed.

Results

The healing was uneventful with no early or delayed complications. The implants were loaded using a standard loading protocol with porcelain fused to metal crowns. The patients were placed on a 6 month regular recall program. There was no complaint during the recall period of 2 years.

Conclusions

Within the limits of the current case series it can be concluded that short implants might be an alternative in cases needing explantation, at least for the short term of 2 years.

Funding source(s)

n/a

Sažetak

Naslov

Short implants as an alternative treatment for areas of implant explantation.

Objective

Methods

Three patients complaining of implant related problems referred to Ege University, Faculty of Dentistry, Department of Periodontology. The patients were male, in their 50's, systemically healthy with no allergies or other medical condition. All implants were in function for more than 10 years and currently had excessive bone loss. One patient had two periimplantitis implants and one implant breakage, other patient had one periimplantitis and the other patient had a broken implant. All the implants were explanted and after a healing period of 6 week one short implant for each explanted implant were placed.

Results

The early healing was uneventful with no early or delayed complications. The implants were loaded using a standard loading protocol with porcelain fused to metal crowns. The patients were placed on a 6 month regular recall program. There was no complaint during the follow up period of 2 years.

Conclusions

Within the limits of the current case series it can be concluded that short implants might be an alternative in cases needing explantation, at least for the short term of 2 years.

Funding source(s)

n/a

Sažetak

Naslov

Gingival enlargement following cardiac transplant surgery in 44 year old male patient

Objective

Methods

Patient underwent non-surgical periodontal therapy. The treatments included scaling, root planing and oral hygiene instruction. Patient is a nonsmoker.

Results

After 3-months of follow-up the results were satisfactory and stable. There was no further gingival enlargement visible.

Conclusions

Clinical approach should focus on eliminating infection sources and creating an oral environment that facilitates easy plaque control. Through proper diagnosis and treatment combined with good patient cooperation there was no need for surgical therapy. As additional therapy patient used antiseptic mouthwash (chlorhexidine 0.12 %) and Amlopine supstitution was recommended with similar action and no gigngival growth stimulation.

Funding source(s)

Khocht A, Schneider LC. Periodontal management of gingival overgrowth in the heart transplant patient: a case report. J Periodontol. 1997 Nov;68(11):1140-6. doi: 10.1902/jop.1997.68.11.1140. PMID: 9407409. Pundir AJ, Pundir S, Yeltiwar RK, Farista S, Gopinath V, Srinivas TS. Treatment of drug-induced gingival overgrowth by full-mouth disinfection: A non-surgical approach. J Indian Soc Periodontol. 2014 May;18(3):311-5. doi: 10.4103/0972-124X.134567. PMID: 25024543; PMCID: PMC4095622.

Sažetak

Naslov

Evaluation of Gingival Crevice Fluid Annexin-A1 Levels in Periodontal Diseases

Objective

Methods

In total 80 systemically healthy individuals were included in this study; 20 with periodontitis stage 3 grade B (P-Stage III/B), 20 with periodontitis stage 3 grade C (P-Stage III/C), 19 with gingivitis, and 21 with clinically healthy periodontium (H). Probing depth, clinical attachment level, plaque index and papillary bleeding index were recorded. GCF AnxA1 levels were analyzed by enzyme-linked immunosorbent assay. Non-parametrical tests were used for statistical comparisons.

Results

All clinical parameters were significantly higher in periodontitis patients compared to healthy individuals (p<0.008). P-Stage III/B, P-Stage III/C, gingivitis and healthy groups had similar GCF AnxA1 total amount (p<0.008). GCF AnxA1 negatively correlated with the all clinical parameters (p<0.05).

Conclusions

Low GCF AnxA1 levels in periodontitis might point to the failure of AnxA1 in controlling the local inflammatory response and resolving inflammation and to the possible role of AnxA1 in the pathogenesis of periodontitis.

Funding source(s)

N/A

Sažetak

Naslov

Gene Expression in Implants: Nano-Hydroxyapatite and L-PRF Impact on Osseointegration in Osteoporotic Rat Model

Objective

Methods

One implant of each surface was surgically placed in the right tibia of sham and osteoporotic female rats. Both groups received L-PRF. They were euthanized at 7 and 30 days. The peri-implant bone was harvested to investigate the mRNA expression (Alp, Runx2, Opn, Rank) by real-time PCR.

Results

The presence of L-PRF in sham-Nano group increased Alp mRNA at 30 days (Alp (sham-Nano + L-PRF): 10.11 ± 2.66 versus Alp (sham-Nano): 4.01 ± 2.66). The L-PRF also increased Alp, Runx2, Opn and Rank mRNA at 30 days only in the osteoporotic-Nano group (Alp (osteoporotic-Nano + L-PRF): 11.69 ± 3.90 versus Alp (osteoporotic-Nano): 3.25 ± 3.56, Runx2 (osteoporotic-Nano + L-PRF): 4.49 ± 2.79 versus Runx2 (osteoporotic-Nano): 0.08 ± 2.79; Opn (osteoporotic-Nano + L-PRF): 6.76 ± 2.31 versus Opn (osteoporotic-Nano): 1.90 ± 2.54; Rank (osteoporotic-Nano + L-PRF): 9.06 ± 4.12 versus Rank (osteoporotic-Nano): 0.01 ± 2.91).

Conclusions

These findings suggest that combining nano-hydroxyapatite and L-PRF may improve healing and osseointegration, enhancing dental implant rehabilitation outcomes.

Funding source(s)

São Paulo Research Foundation—FAPESP (Grant 2019/04468-3) and CNPq (National Council for Scientific and Technological Development) Grant 153247/2018-5.

Sažetak

Naslov

Free gingival graft after excision of gingival papilloma

Objective

Methods

A clinical examination of a 12-year-old female patient revealed the existence of a clearly limited formation with a wide base connected to the attached gingiva in the area of tooth 11. At the control examination after supragingival instrumentation, the measured PPD was 4 mm at vestibular site, with negative BOP and plaque index. The operation was performed under local anesthesia, during which excision and fenulectomy were immediately performed. A free gingival graft was taken from area of the hard palate and sewn to the place of the removed change. Instructions were given regarding the proper maintenance of oral hygiene.

Results

After the procedure, healig was successfully healed. The sample was sent for pathohistological diagnosis, which showed that it was part of a papilloma.

Conclusions

By transplanting a free gingival graft at the site of removed formation, the existing gingival recession was partially covered and thus created the band of lost attached gingiva, with satisfactory aesthetic result.

Funding source(s)

n/a

Sažetak

Naslov

Covering of gingival recessions with Coronally advanced flap surgical technique by Zucchelli, using hyaluronic acid

Objective

Methods

Analysis of the patient’s orthopantomogram, as well as the measured indexes, showed no signs of periodontitis. The recessions were 3 mm on teeth 21 and 22, and 4 mm on tooth 23. The width of the attached gingiva was 4 mm on tooth 21, 5 mm on tooth 22, and 2.5 mm on tooth 23. The operation was performed under local anesthesia, exposed parts of root surfaces were instrumented with a curettes and treated for a period of two minutes with EDTA gel, and hyaluronic acid was applied before suturing.

Results

At the follow-up examination after 14 days healing was successfully, without subjective disturbances. A control examination after 3 and 6 months showed clinical stability and expansion of the attached gingival zone.

Conclusions

With this procedure recessions can be successfully covered on all teeth in upper and lower jaw, as the technique shows excellent long-term aesthetic results.

Funding source(s)

n/a

Sažetak

Naslov

Expanding the band of attached gingiva around the implant using a free gingival graft transplant

Objective

Methods

In a 76-year-old patient, a clinical examination revealed the absence of a band of attached gingiva around the implant in the area of the extracted tooth 44. The analysis of the retroalveolar dental image as well as the measured indices did not show any signs of periimplatitis. After supragingival instrumentation was performed, the operation is performed under local anesthesia, during which the free gingival graft is taken from the area of the hard palate and fixed with non-absorbable monofilament sutures. Postoperative care included rinsing with 0.2% chlorhexidine solution, while avoiding brushing the operated region.

Results

At the clinical examination 14 days after procedure healing was successfully with no signs of inflammation.

Conclusions

At the follow-up examination 3 months after the procedure, the width of the band of attached gingiva was 3 mm, and the peri-implant mucosa shows great stability .

Funding source(s)

n/a

Sažetak

Naslov

Periodontal status of IQOS tobacco heating device smokers and cigarette smokers

Objective

Methods

The research was conducted on 66 patients of the KBC Rijeka, Clinic of dental medicine. Patients were divided into three groups: non-smokers, classic cigarettes smokers and IQOS smokers. The procedure involved gathering of the anamnestic data, clinical examination and measurement of periodontal indices: PPD, GR, TM, FD, CAL, FMPS and FMBS.

Results

There is a difference in CAL and average PPD between cigarette smokers and non-smokers, as well as between IQOS smokers and cigarette smokers in CAL, but not in PPD. No difference was observed in any of the periodontal indices between the non-smokers and IQOS users. Non-smokers and IQOS smokers had a lower number and lower proportion of teeth with deep periodontal pockets (≥ 6 mm), compared to the group of cigarette smokers.

Conclusions

IQOS use demonstrated a less harmful effect on the periodontal status measured by the periodontal indices PPD and CAL compared to classic cigarettes. No significant difference has been found for PPD and CAL values between non-smokers and IQOS smokers.

Funding source(s)

The research is part of the project "Environmental factors and microbiological interactions in the structure of the dental biofilm financed" led by Prof. Ph.D. Stjepan Špalj and was financed by HZZZ IP-2020-02-4027.

Sažetak

Naslov

Salivary Mediators Involving in the Regulation of Transendothelial Migration of Neutrophils in Grade C Periodontitis

Objective

Methods

A total of 40 systemically healthy non-smokers(Clinical Health n=20,Grade C Periodontitis n=20) were included in the study.Clinical periodontal records and unstimulated saliva samples were collected.Cytokine levels were evaluated by ELISA method. A Mann Whitney U test(unpaired Wilcoxon test) was run to determine whether there was a statistically significant difference between groups according to the biochemical parameters.

Results

Considering the biochemical parameters, the LFA-1 level was significantly higher in the Grade C Periodontitis group compared to the Clinical Health (p=.009).On the other hand, we did not observe statistically significant difference between groups of salivary PTX-3, GDF-15, P-Selectin, VCAM, ICAM, and Del-1 levels.

Conclusions

According to study, LFA-1 is more active player during transendothelial migration.Increased inflammatory response might be related to the higher LFA-1 levels in Grade C periodontitis.Further studies with higher number of participants with different grading and staging are required to clarify the pathogenesis of periodontitis.

Funding source(s)

n/a

Sažetak